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Application pool failed to respond to a ping

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Hi there,

Just encounter a strange problem that windows event logs sometimes show Application pool failed to respond to a ping, probablely 1 or 2 times a day or once a week. It would automatically restore within few minutes. Not always happened in specific web application code, others code would happen too. The same code in other machine(same OS、IIS、ASP、MVC) almost not found this problem.

The AppPool recycle every am 12:00. idleTimeoute=60, idleTimeoutAction=suspend.
The IIS logs all show sc-status 200, but time-taken is long(about 25000ms) then usual(within 2000ms).
The Http.sys logs show connection_abandoned_by_reqqueue. 

Does this can only use debug diagnostic tools to debug? Thank you all.

OS: server 2012 r2 standard

IIS:8.5

ASP.NET: 4.0.30319

MVC:5.2.3


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