Classic ASP: Internal Server Error 500

I am getting an Internal server error 500 on first approach to my site. Then if you refresh it will launch the site correctly. I am still trying to figure out what the problem is whether it is IIS or the code on the index page.

The log has the follow line

2013-12-09 15:29:00 xxx.x.x.x GET / |37|80070005|Access_is_denied.__ 80 - x.x.x.x Mozilla/5.0+(Windows+NT+6.1;+WOW64)+AppleWebKit/537.36+(KHTML,+like+Gecko)+Chrome/31.0.1650.63+Safari/537.36 500 0 0 702

Thanks for any help.