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HTTP Error 503. The service is unavailable

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Hi,

Suddenly we started  getting HTTP Error 503. The service is unavailable for a IIS 7 Production Website.   

1. Website only works when Application Pool Identity is set to "Network Services" but didnot work when set as custom identity

2. Local User which which is used as custom identity , is member of IIS_IUSRS group and also member of Administrator group.

3. IIS_IUSRS and Custom Identity user have Read access to the web directory and Administrator have full access.

I am almost sure its is permission/privilege issue of IIS_IUSRs group but cant find what is missing and where ? Plesae help.

httperr Logs

2013-11-27 07:24:30 127.0.0.1 60821 127.0.0.1 80 HTTP/1.1 GET / 503 4 AppOffline testsite
2013-11-27 07:25:26 127.0.0.1 60822 127.0.0.1 80 HTTP/1.1 GET / 503 4 AppOffline testsite
2013-11-27 07:27:17 127.0.0.1 60825 127.0.0.1 80 HTTP/1.1 GET / 503 4 AppOffline testsite

Web Access logs

#Date: 2013-11-27 07:12:12
#Fields: date time s-ip cs-method cs-uri-stem cs-uri-query s-port cs-username c-ip cs(User-Agent) sc-status sc-substatus sc-win32-status time-taken
2013-11-27 07:12:12 127.0.0.1 GET / - 80 - 127.0.0.1 Mozilla/4.0+(compatible;+MSIE+8.0;+Windows+NT+6.1;+WOW64;+Trident/4.0;+SLCC2;+.NET+CLR+2.0.50727;+.NET
+CLR+3.5.30729;+.NET+CLR+3.0.30729;+.NET4.0C;+.NET4.0E) 304 0 0 140
2013-11-27 07:12:12 127.0.0.1 GET /welcome.png - 80 - 127.0.0.1 Mozilla/4.0+(compatible;+MSIE+8.0;+Windows+NT+6.1;+WOW64;+Trident/4.0;+SLCC2;+.NET+CLR
+2.0.50727;+.NET+CLR+3.5.30729;+.NET+CLR+3.0.30729;+.NET4.0C;+.NET4.0E) 304 0 0 15
2013-11-27 07:13:42 127.0.0.1 GET / - 80 - 127.0.0.1 Mozilla/4.0+(compatible;+MSIE+8.0;+Windows+NT+6.1;+WOW64;+Trident/4.0;+SLCC2;+.NET+CLR+2.0.50727;+.NET
+CLR+3.5.30729;+.NET+CLR+3.0.30729;+.NET4.0C;+.NET4.0E) 304 0 0 0
2013-11-27 07:13:42 127.0.0.1 GET /welcome.png - 80 - 127.0.0.1 Mozilla/4.0+(compatible;+MSIE+8.0;+Windows+NT+6.1;+WOW64;+Trident/4.0;+SLCC2;+.NET+CLR
+2.0.50727;+.NET+CLR+3.5.30729;+.NET+CLR+3.0.30729;+.NET4.0C;+.NET4.0E) 304 0 0 0

Event Viewer Application LOgs

Log Name: Application
Source: Microsoft-Windows-IIS-W3SVC-WP
Date: 27/11/2013 3:38:13 PM
Event ID: 2276
Task Category: None
Level: Error
Keywords: Classic
User: N/A
Computer: testwebserver
Description:
The worker process failed to initialize correctly and therefore could not be started. The data is the error.
Event Xml:
<Event xmlns="http://schemas.microsoft.com/win/2004/08/events/event">
<System>
<Provider Name="Microsoft-Windows-IIS-W3SVC-WP" Guid="{670080D9-742A-4187-8D16-41143D1290BD}" EventSourceName="W3SVC-WP" />
<EventID Qualifiers="49152">2276</EventID>
<Version>0</Version>
<Level>2</Level>
<Task>0</Task>
<Opcode>0</Opcode>
<Keywords>0x80000000000000</Keywords>
<TimeCreated SystemTime="2013-11-27T07:38:13.000000000Z" />
<EventRecordID>18904</EventRecordID>
<Correlation />
<Execution ProcessID="0" ThreadID="0" />
<Channel>Application</Channel>
<Computer>testwebserver</Computer>
<Security />
</System>
<EventData>
<Binary>05000780</Binary>
</EventData>
</Event>

Event Viewer- System Logs

Log Name: System
Source: Microsoft-Windows-WAS
Date: 27/11/2013 3:38:13 PM
Event ID: 5002
Task Category: None
Level: Error
Keywords: Classic
User: N/A
Computer: testwebserver
Description:
Application pool 'testsite' is being automatically disabled due to a series of failures in the process(es) serving that application pool.
Event Xml:
<Event xmlns="http://schemas.microsoft.com/win/2004/08/events/event">
<System>
<Provider Name="Microsoft-Windows-WAS" Guid="{524B5D04-133C-4A62-8362-64E8EDB9CE40}" EventSourceName="WAS" />
<EventID Qualifiers="49152">5002</EventID>
<Version>0</Version>
<Level>2</Level>
<Task>0</Task>
<Opcode>0</Opcode>
<Keywords>0x80000000000000</Keywords>
<TimeCreated SystemTime="2013-11-27T07:38:13.000000000Z" />
<EventRecordID>53187</EventRecordID>
<Correlation />
<Execution ProcessID="0" ThreadID="0" />
<Channel>System</Channel>
<Computer>testwebserver</Computer>
<Security />
</System>
<EventData>
<Data Name="AppPoolID">testsite</Data>
<Binary>
</Binary>
</EventData>
</Event>


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