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How to resolve the issue of Integrated windows authentication asking username and password in Windows Server 2008 R2 , IIS 7.5 ?

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I had server Windows Server 2008 R2, IIS 7.5 

 WebSite has to be accessed from Intranet by authenticated users of different domains.

Website is running under AppPool custom account

Only Integrated windows authenitcation is enabled, rest every thing is disabled mode.

We had tried resolution steps

1) given full Folder permissions for the custom account of AppPool

Not Authorized

HTTP Error 401. The requested resource requires user authentication

Server Error

401 - Unauthorized: Access is denied due to invalid credentials.

You do not have permission to view this directory or page using the credentials that you supplied.

When website is accessed by users, for many of users, it is working fine,

for some users, it is asking to enter credentials,

How to solve this problem..?

we tried in client machine browser, internet options-->Secuirty -->Disable integrated windows authentication

with this for some users problem resolved, but they are still some users who are not able to acess the site,

even though enter credntials, for second time , some can access site, for some even 3 times asking, after that it throws error ?

please help me to resolve the isse .

thanks for your help in advance ......

Thanks

Rama


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