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asp net initialization exception module 500 error

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I am using Windows Server 2008 R2 with IIS 7.5 Installed. It already has one website running on ASP. Can I run ASP.NET using another application pool and required ASP.NET Framework. Tried every method but it is giving this error.(Details in image link attached). Thanks in advance if anyone has any idea.

P.s. I have tried every steps shown on different forum on internet still no luck. If you have any idea please do share.

My only question. Is it because this new website using ASP.NET framework is conflicting with existing ASP website. Can we run two websites simultaneously in to same server in IIS!?

Error 500 Image Detailed Description


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