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HTTP Error 500.0 - Internal Server Error

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Hello,

I have deployed my application on IIS server and set it as windows authentication and set custom service account in Application Identity pool 

but when i try to run the application Images,JS ,kendo files are not loding it gives me internal server error

<div id="details-left">
ModuleIIS Web Core
NotificationAuthenticateRequest
HandlerStaticFile
Error Code0x80070542
</div> <div id="details-right">
Requested URL
Physical Path
Logon MethodNegotiate
Logon UserGLOBAL\******

but when i open browser with service account provided  then it works fine no issue

I tried everything adding IIS_users,service account  and giving permission to everyone to that directory. but not working for me 

it works only when  i login with user that i have set in application pool identity.

Thanks and regards

Vaibhav Pawar

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