Error -2147024891 Access Denied Launch Excel from Web App (Python)

Hello there:

I have a problem between IIS, Python and Excel, I can do everything with the IDE (visual studio) but when i do the same thing with the IIS web app the result is:

    IDispatch = pythoncom.CoCreateInstance(IDispatch, None, clsctx, pythoncom.IID_IDispatch)
com_error: (-2147024891, 'Access is denied.', None, None)

Step i've already done:

-permission for write and read on C:\inetpub\wwwroot\MyWebSite (subfolders included)

-i checked the DCOM Excel permision,

i have some doubts about the webapp settings on the IIS manager, i have to add a webapplication (path and give a alias, etc?) and grant permissions to this app/folder?

On python i have to change something? the task is very simply: open the Excel Run the macro and that's it.

import os
from MyAPP import app
from flask import Flask, render_template, request, redirect, url_for, send_from_directory, after_this_request
from werkzeug import secure_filename
from win32com.client import Dispatch
import pythoncom 
import upload

from flask import send_file
import StringIO


@app.route('/testing') 
def test():
    mc_test= 'Test_macro.Prueba'
    mc_test_run= r'C:\inetpub\wwwroot\MyWebSite\MyApp\Test.xlsm'
    run_macro(mc_test, mc_test_run) 
    return 'macro done!'

def run_macro(macroname, filetorun):
            pythoncom.CoInitialize ()
            xlApp = Dispatch('Excel.Application')
            xlWb = xlApp.Workbooks.Open(filetorun)
            xlSht = xlWb.WorkSheets(1)
            xlApp.visible = True
            xlApp.Application.Run(macroname)
            xlWb.Saved = 0
            xlWb.Save()
            xlWb.Close(SaveChanges=True)
            xlApp.application.Quit()

            

that's the code in few words, nothing special.

So, i need to allow python to execute the excel macro when the user enter to the webapp site or press the button.

Thanks in advance.

pd: i have Windows 8.1 and using python 2.7